LOCUS       pYPKa_Z_TEF1            3720 bp    DNA     circular UNK 14-JUL-2015
DEFINITION  cSEGUID_0s47KRvklrl6IzfaACskS5ZBWPM_2015-07-22T17:28:25.474112
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc            531..554
                     /label="TEF1fw"
     primer_bind     537..554
                     /note="pfw579"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(1094..1115)
                     /note="prv579"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1094..1122)
                     /label="TEF1rv"
     primer_bind     complement(1150..1170)
                     /note="567_pCAPsAjiIF"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1150..1172)
                     /label="567_pCAPsAjiIF"
     misc            1173..1194
                     /label="568_pCAPsAjiIR"
     primer_bind     1175..1194
                     /note="568_pCAPsAjiIR"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     rep_origin      1905
                     /direction=BOTH
     gene            complement(2664..3524)
                     /gene="bla"
     CDS             complement(2664..3524)
                     /product="beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="GI:2769263"
                     /db_xref="GOA:Q79DR3"
                     /db_xref="HSSP:P62593"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /db_xref="UniProtKB/TrEMBL:Q79DR3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /gene="bla"
                     /protein_id="CAA04868.1"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaatacaa
      541 tgcatacttt gtacgttcaa aatacaatgc agtagatata tttatgcata ttacatataa
      601 tacatatcac ataggaagca acaggcgcgt tggactttta attttcgagg accgcgaatc
      661 cttacatcac acccaatccc ccacaagtga tcccccacac accatagctt caaaatgttt
      721 ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa
      781 aacacccaag cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta
      841 cccgtactaa aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa
      901 aaaggcaata aaaattttta tcacgtttct ttttcttgaa aatttttttt tttgattttt
      961 ttctctttcg atgacctccc attgatattt aagttaataa acggtcttca atttctcaag
     1021 tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga
     1081 aagcatagca atctaatcta agttttaatt acaaattaat tagtcgagga acgccaggtt
     1141 gcccactttc tcactagtga cctgcagccg acgtgccatc tgtgcagaca aacgcatcag
     1201 gatatccgga tttacctgaa tcaattggcg aaattttttg tacgaaattt cagccacttc
     1261 acaggcggtt ttcgcacgta cccatgcgct acgttcctgg ccctcttcaa acaggcccag
     1321 ttcgccaata aaatcaccct gattcagata ggagaggatc atttctttac cctcttcgtc
     1381 tttgatcagc actgccacag agcctttaac gatgtagtac agcgtttccg ctttttcacc
     1441 ctggtgaata agcgtgctct tggatgggta cttatgaatg tggcaatgag acaagaacca
     1501 ttcgagagta ggatccgttt gaggtttacc aagtaccata agatccttaa atttttatta
     1561 tctagctaga tgataatatt atatcaagaa ttgtacctga aagcaaataa attttttatc
     1621 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     1681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2581 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgctaag
     3661 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//