LOCUS       pYPK0_tp_g_tp           8024 bp    DNA     circular UNK 14-JUL-2015
DEFINITION  pYPK0_TEF1_SsXYL1_TDH3
            cSEGUID_M2q6_Lxm8JLLUJDAn3vRRNk5KtA_2015-07-22T17:28:39.758573
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     overlap         363..486
                     /note="olp_GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_revcolor="#BAD4F0"
                     /chksum="GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_fwdcolor="#CCF6E4"
     primer_bind     complement(458..486)
                     /note="865_pYPKpwR"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            531..554
                     /label="TEF1fw"
     primer_bind     537..554
                     /note="pfw579"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(1094..1115)
                     /note="prv579"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1094..1122)
                     /label="TEF1rv"
     overlap         1123..1172
                     /note="olp_8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_revcolor="#E1D3DC"
                     /chksum="8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_fwdcolor="#BBF5D5"
     primer_bind     complement(1150..1170)
                     /note="567_pCAPsAjiIF"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1150..1172)
                     /label="567_pCAPsAjiIF"
     primer_bind     complement(1150..1172)
                     /note="567"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1150..1172)
                     /label="567"
     misc            1173..1194
                     /label="SsXYL1fw"
     primer_bind     1175..1194
                     /note="pfw957"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     complement(2111..2131)
                     /note="prv957"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(2111..2131)
                     /label="SsXYL1rv"
     misc            2132..2153
                     /label="568_pCAPsAjiIR"
     overlap         2132..2168
                     /note="olp_-ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_revcolor="#CBB2C1"
                     /chksum="-ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_fwdcolor="#F3EEF3"
     primer_bind     2134..2153
                     /note="568_pCAPsAjiIR"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     complement(2138..2162)
                     /note="467"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(2138..2168)
                     /label="467"
     primer_bind     2169..2187
                     /note="pfw698"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     complement(2847..2866)
                     /note="prv698"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(2847..2873)
                     /label="TDH3rv"
     overlap         2927..3168
                     /note="olp_WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_revcolor="#FFEEEB"
                     /chksum="WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_fwdcolor="#D2F6F1"
     primer_bind     complement(3140..3168)
                     /note="578"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(3140..3168)
                     /label="578"
     rep_origin      3575
                     /ApEinfo_fwdcolor="cyan"
                     /direction=BOTH
                     /label="feat1"
                     /ApEinfo_revcolor="red"
     misc            complement(4319..5122)
                     /ApEinfo_fwdcolor="cyan"
                     /label="URA3"
                     /ApEinfo_revcolor="red"
     CDS             complement(6971..7629)
                     /ApEinfo_fwdcolor="cyan"
                     /label="AmpR"
                     /ApEinfo_revcolor="red"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaatacaa
      541 tgcatacttt gtacgttcaa aatacaatgc agtagatata tttatgcata ttacatataa
      601 tacatatcac ataggaagca acaggcgcgt tggactttta attttcgagg accgcgaatc
      661 cttacatcac acccaatccc ccacaagtga tcccccacac accatagctt caaaatgttt
      721 ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa
      781 aacacccaag cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta
      841 cccgtactaa aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa
      901 aaaggcaata aaaattttta tcacgtttct ttttcttgaa aatttttttt tttgattttt
      961 ttctctttcg atgacctccc attgatattt aagttaataa acggtcttca atttctcaag
     1021 tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga
     1081 aagcatagca atctaatcta agttttaatt acaaattaat tagtcgagga acgccaggtt
     1141 gcccactttc tcactagtga cctgcagccg acaaatgcct tctattaagt tgaactctgg
     1201 ttacgacatg ccagccgtcg gtttcggctg ttggaaagtc gacgtcgaca cctgttctga
     1261 acagatctac cgtgctatca agaccggtta cagattgttc gacggtgccg aagattacgc
     1321 caacgaaaag ttagttggtg ccggtgtcaa gaaggccatt gacgaaggta tcgtcaagcg
     1381 tgaagacttg ttccttacct ccaagttgtg gaacaactac caccacccag acaacgtcga
     1441 aaaggccttg aacagaaccc tttctgactt gcaagttgac tacgttgact tgttcttgat
     1501 ccacttccca gtcaccttca agttcgttcc attagaagaa aagtacccac caggattcta
     1561 ctgtggtaag ggtgacaact tcgactacga agatgttcca attttagaga cctggaaggc
     1621 tcttgaaaag ttggtcaagg ccggtaagat cagatctatc ggtgtttcta acttcccagg
     1681 tgctttgctc ttggacttgt tgagaggtgc taccatcaag ccatctgtct tgcaagttga
     1741 acaccaccca tacttgcaac aaccaagatt gatcgaattc gctcaatccc gtggtattgc
     1801 tgtcaccgct tactcttcgt tcggtcctca atctttcgtt gaattgaacc aaggtagagc
     1861 tttgaacact tctccattgt tcgagaacga aactatcaag gctatcgctg ctaagcacgg
     1921 taagtctcca gctcaagtct tgttgagatg gtcttcccaa agaggcattg ccatcattcc
     1981 aaagtccaac actgtcccaa gattgttgga aaacaaggac gtcaacagct tcgacttgga
     2041 cgaacaagat ttcgctgaca ttgccaagtt ggacatcaac ttgagattca acgacccatg
     2101 ggactgggac aagattccta tcttcgtcta agtgccatct gtgcagacaa acgcatcagg
     2161 atttaaatat aaaaaacacg ctttttcagt tcgagtttat cattatcaat actgccattt
     2221 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
     2281 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
     2341 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
     2401 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
     2461 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
     2521 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
     2581 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
     2641 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
     2701 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     2761 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
     2821 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaattaa ttaatccgga
     2881 tttacctgaa tcaattggcg aaattttttg tacgaaattt cagccacttc acaggcggtt
     2941 ttcgcacgta cccatgcgct acgttcctgg ccctcttcaa acaggcccag ttcgccaata
     3001 aaatcaccct gattcagata ggagaggatc atttctttac cctcttcgtc tttgatcagc
     3061 actgccacag agcctttaac gatgtagtac agcgtttccg ctttttcacc ctggtgaata
     3121 agcgtgctct tggatgggta cttatgaatg tggcaatgag acaagaacca ttcgagagta
     3181 ggatccgttt gaggtttacc aagtaccata agatccttaa atttttatta tctagctaga
     3241 tgataatatt atatcaagaa ttgtacctga aagcaaataa attttttatc tggcttaact
     3301 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3361 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     3421 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3481 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3601 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3661 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3721 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3781 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3841 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3901 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3961 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4021 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4081 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4201 agtggaacga aaactcacgt taagggattt tggtcatgag gggtaataac tgatataatt
     4261 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
     4321 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
     4381 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
     4441 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
     4501 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
     4561 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
     4621 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
     4681 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
     4741 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
     4801 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
     4861 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
     4921 aatcagtcaa aatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
     4981 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
     5041 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
     5101 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca
     5161 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     5221 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     5281 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt
     5341 gaaaagctag cttatcgatg ataagctgtc aaagatgaga attaattcca cggactatag
     5401 actatactag atactccgtc tactgtacga tacacttccg ctcaggtcct tgtcctttaa
     5461 cgaggcctta ccactctttt gttactctat tgatccagct cagcaaaggc agtgtgatct
     5521 aagattctat cttcgcgatg tagtaaaact agctagaccg agaaagagac tagaaatgca
     5581 aaaggcactt ctacaatggc tgccatcatt attatccgat gtgacgctgc agcttctcaa
     5641 tgatattcga atacgctttg aggagataca gcctaatatc cgacaaactg ttttacagat
     5701 ttacgatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     5761 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     5821 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     5881 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     5941 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     6001 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     6061 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gacgagagcg
     6121 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     6181 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     6241 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     6301 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     6361 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     6421 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     6481 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     6541 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     6601 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     6661 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     6721 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     6781 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atgggaagct ccaccccggt
     6841 tgataatcag aaaagcccca aaaacaggaa gattattatc aaaaaggatc ttcacctaga
     6901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag cgcttaccat
     7081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7261 tgcgcaacgt tgttggcatt gctacaggca tcgtggtgtc actctcgtcg tttggtatgg
     7321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7561 cgagttgctc ttgcccggcg tcaatacggg ataatagtgt atcacatagc agaactttaa
     7621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7921 taggggttcc gcgcacattt ccccgaaaag tgccacctgc taagaaacca ttattatcat
     7981 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//