LOCUS       pYPKa_Z_PDC1            4096 bp    DNA     circular UNK 14-JUL-2015
DEFINITION  cSEGUID_nRul67Jr3-VMjnL1l2kF5dPDvcI_2015-07-22T17:28:18.138250
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc            531..552
                     /label="PDC1fw"
     primer_bind     537..552
                     /note="pfw955"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(1474..1491)
                     /note="prv955"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1474..1498)
                     /label="PDC1rv"
     primer_bind     complement(1526..1546)
                     /note="567_pCAPsAjiIF"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1526..1548)
                     /label="567_pCAPsAjiIF"
     misc            1549..1570
                     /label="568_pCAPsAjiIR"
     primer_bind     1551..1570
                     /note="568_pCAPsAjiIR"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     rep_origin      2281
                     /direction=BOTH
     gene            complement(3040..3900)
                     /gene="bla"
     CDS             complement(3040..3900)
                     /product="beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="GI:2769263"
                     /db_xref="GOA:Q79DR3"
                     /db_xref="HSSP:P62593"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /db_xref="UniProtKB/TrEMBL:Q79DR3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /gene="bla"
                     /protein_id="CAA04868.1"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaataggg
      541 tagcctcccc ataacataaa ctcaataaaa tatatagtct tcaacttgaa aaaggaacaa
      601 gctcatgcaa agaggtggta cccgcacgcc gaaatgcatg caagtaacct attcaaagta
      661 atatctcata catgtttcat gagggtaaca acatgcgact gggtgagcat atgttccgct
      721 gatgtgatgt gcaagataaa caagcaaggc agaaactaac ttcttcttca tgtaataaac
      781 acaccccgcg tttatttacc tatctctaaa cttcaacacc ttatatcata actaatattt
      841 cttgagataa gcacactgca cccatacctt ccttaaaaac gtagcttcca gtttttggtg
      901 gttccggctt ccttcccgat tccgcccgct aaacgcatat ttttgttgcc tggtggcatt
      961 tgcaaaatgc ataacctatg catttaaaag attatgtatg ctcttctgac ttttcgtgtg
     1021 atgaggctcg tggaaaaaat gaataattta tgaatttgag aacaattttg tgttgttacg
     1081 gtattttact atggaataat caatcaattg aggattttat gcaaatatcg tttgaatatt
     1141 tttccgaccc tttgagtact tttcttcata attgcataat attgtccgct gccccttttt
     1201 ctgttagacg gtgtcttgat ctacttgcta tcgttcaaca ccaccttatt ttctaactat
     1261 ttttttttta gctcatttga atcagcttat ggtgatggca catttttgca taaacctagc
     1321 tgtcctcgtt gaacatagga aaaaaaaata tataaacaag gctctttcac tctccttgca
     1381 atcagatttg ggtttgttcc ctttattttc atatttcttg tcatattcct ttctcaatta
     1441 ttattttcta ctcataacct cacgcaaaat aacacagtca aatcaatcaa attaattagt
     1501 cgaggaacgc caggttgccc actttctcac tagtgacctg cagccgacgt gccatctgtg
     1561 cagacaaacg catcaggata tccggattta cctgaatcaa ttggcgaaat tttttgtacg
     1621 aaatttcagc cacttcacag gcggttttcg cacgtaccca tgcgctacgt tcctggccct
     1681 cttcaaacag gcccagttcg ccaataaaat caccctgatt cagataggag aggatcattt
     1741 ctttaccctc ttcgtctttg atcagcactg ccacagagcc tttaacgatg tagtacagcg
     1801 tttccgcttt ttcaccctgg tgaataagcg tgctcttgga tgggtactta tgaatgtggc
     1861 aatgagacaa gaaccattcg agagtaggat ccgtttgagg tttaccaagt accataagat
     1921 ccttaaattt ttattatcta gctagatgat aatattatat caagaattgt acctgaaagc
     1981 aaataaattt tttatctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2041 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     2101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2701 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4021 agtgccacct gctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4081 acgaggccct ttcgtc
//