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            "Development Status :: 4 - Beta",
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            "License :: OSI Approved :: MIT License",
            "Programming Language :: Python :: 3",
            "Programming Language :: Python :: 3.5",
            "Programming Language :: Python :: 3.6",
            "Programming Language :: Python :: 3.7",
            "Topic :: Scientific/Engineering :: Bio-Informatics"
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        "description": "# Primer3Plus\n\nPrimer3Plus is a Python DNA primer design tool based off of Primer3 and the\nPython primer3 wrapper (https://pypi.org/project/primer3-py/).\n\n```python\nimport json\n\ndesign = Design()\ntemplate = \"\"\"\nTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCAC\nATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAG\nGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTAT\nTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGC\nATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGC\nTCGAAGGCTTTAATTTGC\n\"\"\"\ntemplate = template.replace('\\n', '').replace(' ', '')\ndesign.settings.template(template)\ndesign.settings.as_cloning_task()\ndesign.settings.primer_num_return(1)\nresults, explain = design.run()\n\nprint(json.dumps(results, indent=1))\nprint(json.dumps(explain, indent=1))\n```\n\n```json\n{\n \"0\": {\n  \"PAIR\": {\n   \"PENALTY\": 11.204301707622733,\n   \"COMPL_ANY_TH\": 0.0,\n   \"COMPL_END_TH\": 0.0,\n   \"PRODUCT_SIZE\": 248\n  },\n  \"LEFT\": {\n   \"PENALTY\": 9.027129166714644,\n   \"SEQUENCE\": \"TCATGTAATTAGTTATGTCACGCTTAC\",\n   \"location\": [\n    0,\n    27\n   ],\n   \"TM\": 57.972870833285356,\n   \"GC_PERCENT\": 33.333333333333336,\n   \"SELF_ANY_TH\": 0.0,\n   \"SELF_END_TH\": 0.0,\n   \"HAIRPIN_TH\": 0.0,\n   \"END_STABILITY\": 2.34\n  },\n  \"RIGHT\": {\n   \"PENALTY\": 2.1771725409080886,\n   \"SEQUENCE\": \"GCAAATTAAAGCCTTCGAGCG\",\n   \"location\": [\n    247,\n    21\n   ],\n   \"TM\": 58.82282745909191,\n   \"GC_PERCENT\": 47.61904761904762,\n   \"SELF_ANY_TH\": 0.0,\n   \"SELF_END_TH\": 0.0,\n   \"HAIRPIN_TH\": 38.006257959698985,\n   \"END_STABILITY\": 5.03\n  }\n }\n}\n{\n \"PRIMER_LEFT_EXPLAIN\": \"considered 10, low tm 9, ok 1\",\n \"PRIMER_RIGHT_EXPLAIN\": \"considered 10, low tm 3, high tm 4, ok 3\",\n \"PRIMER_PAIR_EXPLAIN\": \"considered 1, ok 1\",\n \"PRIMER_LEFT_NUM_RETURNED\": 1,\n \"PRIMER_RIGHT_NUM_RETURNED\": 1,\n \"PRIMER_INTERNAL_NUM_RETURNED\": 0,\n \"PRIMER_PAIR_NUM_RETURNED\": 1\n}\n```\n## Installation\n\n```\npip install primer3plus -U\n```\n\n## Requirements\n\npython >= 3.5",
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