{ "info": { "author": "Camille Scott", "author_email": "camille.scott.w@gmail.com", "bugtrack_url": null, "classifiers": [], "description": "dammit!\n=======\n\n.. image:: https://badges.gitter.im/Join%20Chat.svg\n :alt: Join the chat at https://gitter.im/camillescott/dammit\n :target: https://gitter.im/camillescott/dammit?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge\n\n*\"I love writing BLAST parsers!\" -- no one, ever*\n\ndammit is a simple de novo transcriptome annotator. It was born out of the\nobservation that: annotation is mundane and annoying; all the individual pieces\nof the process exist already; and, the existing solutions are overly complicated \nor rely on crappy non-free software. \n\nScience shouldn't suck for the sake of sucking, so dammit attempts\nto make this sucky part of the process suck a little less.\n\nInstallation\n------------\n\nComplete instructions with explanations and more platform options are in the documentation \n`website `__. For the impatient, here's a stripped \ndown version. These instructions assume you're on a clean Ubuntu 14.04 install.\ndammit will run on OSX too, though some of the dependencies will need to be \ninstalled manually and are not included here.\n\nFirst get packages from the Ubuntu archives::\n\n sudo apt-get update\n sudo apt-get install python-pip python-dev python-numpy git ruby hmmer unzip \\\n infernal ncbi-blast+ liburi-escape-xs-perl emboss liburi-perl \\\n build-essential libsm6 libxrender1 libfontconfig1 \\\n parallel\n sudo gem install crb-blast\n\nInstall some packages manually::\n\n cd\n curl -LO https://github.com/TransDecoder/TransDecoder/archive/2.0.1.tar.gz\n tar -xvzf 2.0.1.tar.gz\n cd TransDecoder-2.0.1; make\n export PATH=$HOME/TransDecoder-2.0.1:$PATH\n\n cd\n curl -LO http://last.cbrc.jp/last-658.zip\n unzip last-658.zip\n cd last-658\n make\n export PATH=$HOME/last-658/src:$PATH\n export PATH=$HOME/last-658/scripts:$PATH\n\n cd\n curl -LO http://busco.ezlab.org/v1/files/BUSCO_v1.22.tar.gz \n tar -xvzf BUSCO_v1.22.tar.gz\n chmod +x BUSCO_v1.22/*.py\n export PATH=$HOME/BUSCO_v1.22:$PATH\n cd\n\nTo add these to your environment permanently::\n\n echo 'export PATH=$PATH:$HOME/TransDecoder-2.0.1' >> $HOME/.bashrc\n echo 'export PATH=$PATH:$HOME/last-658/src' >> $HOME/.bashrc\n echo 'export PATH=$HOME/BUSCO_v1.22:$PATH' >> $HOME/.bashrc\n\nNow, install dammit::\n\n sudo pip install -U setuptools\n sudo pip install dammit\n\nThis will spend a bit of time compiling and installing pandas if you don't \nalready have a recent versions installed; the ones available in the Ubuntu 14.04 archives are\njust too old.\n\nDev Version\n~~~~~~~~~~~\n\nIf you want the latest features (and bugs), you can install dammit from github::\n\n pip install git+https://github.com/camillescott/dammit.git\n\nUsage\n-----\n\nTo check for dependencies, run::\n\n dammit dependencies\n\nTo check for databases, run::\n\n dammit databases\n\nand to download and install them, run::\n\n dammit databases --install\n\nTo annotate your transcriptome, the most basic usage is::\n\n dammit annotate \n\nThese are extremely basic examples; for a much more detailed description, take a look at the\nrelevant page in the `documentation `__. The\ndocumentation describes how to customization the database installation location and utilize existing\ndatabases.\n\nKnown Issues\n------------\n\n* On some systems, installation of the ConfigParser package can get borked, which will cause\n and exception to be thrown. This can be fixed by following the directions at issue #33: https://github.com/camillescott/dammit/issues/33.\n* There can be errors resuming runs which were interrupted on the BUSCO stage. If the task fails on\n resume, delete the BUSCO results folder within your dammit results folder, which will have a name\n of the form `run_.busco_results`.\n* The `dependencies` subcommand doesn't search for all subdependencies; for example, BUSCO relies on\n EMBOSS, which is not searched for. 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