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        "author": "Mark Teese and Philipp Heckmeier",
        "author_email": "mark.teese@checkmytumhomepage.de",
        "bugtrack_url": null,
        "classifiers": [
            "Intended Audience :: Science/Research",
            "License :: OSI Approved :: MIT License",
            "Programming Language :: Python :: 3.6",
            "Topic :: Scientific/Engineering :: Chemistry",
            "Topic :: Scientific/Engineering :: Physics"
        ],
        "description": "==========\nblitzcurve\n==========\n\nData analysis of time-resolved fluorescence anisotropy measurements (TRAMs).\n\nTRAMs are state-of-the-art techniques that can be used to analyse protein function and interaction.\n\nWhy use TRAM techniques?\n~~~~~~~~~~~~~~~~~~~~~~~~\n\nTo measure molecule rotation speeds (e.g. protein size, structure, ligand binding).\n\nTo measure oligomerisation properties, via Foerster Resonance Energy Transfer (FRET) between two fluorescent molecules.\n\nWhat is blitzcurve for?\n~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n\n* fitting curves to experimental TRAM data\n* extracting useful fit parameters\n* comparing samples\n\nHow does the experiment work?\n~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n\n* excitation of fluorescent molecules\n* measurement of the depolorisation of the emitted light (polarisation / anisotropy)\n* time-resolved methods: pulse excitation, and measurement of the change in anisotropy over time in nanoseconds\n\nAnalysis methods\n~~~~~~~~~~~~~~~~\n\n* appropriate fitting methods are still under development\n* current input: csv with anisotropy and time (ns) values\n* current fitting methods for anisotropy vs time\n   - Savitzky Golay fit to all raw data\n   - Exponential fit to initial decay data\n   - Exponential fit to final decay data\n* key measured parameters:\n   - r_inf (predicted anisotropy at an infinite range in time)\n   - r_max (maximum anisotropy measured at any timepoint)\n\nInstallation\n~~~~~~~~~~~~\n::\n\n    pip install blitzcurve\n\n* Blitzcurve should be compatable with `Anaconda python`__ 3.x or any scientific python package\n\n.. _AnacondaLink: https://www.anaconda.com/download/\n\n__ AnacondaLink_\n\nUsage\n~~~~~\n\n.. code:: python\n\n    import blitzcurve\n    # define data directory with csv files\n    data_dir = r\"D:\\data\\20180229_TRdata\"\n    # OPTIONAL: define which data files will be analysed\n    file_list = [\"10nM-FGC1-2min_aniso.txt\", \"10nM-FGC2-2min_aniso.txt\"]\n    # run blitzcurve function to fit curves to individual samples\n    blitzcurve.run_fit(data_dir, figs_to_plot=file_list)\n    # setup a dictionary to shorten long sample names\n    name_dict = {\"10nM-FGC1-2min_aniso.txt\": \"FGC1\", \"10nM-FGC2-2min_aniso.txt\": \"FGC2\", \"10nM-FGC3-2min_aniso.txt\": \"FGC3\"}\n    # run blitzcurve function to compare curves and parameters for multiple samples\n    blitzcurve.run_compare(data_dir, name_dict=name_dict)\n\nContribute\n~~~~~~~~~~\n\nCollaborators and pull requests are welcome. Send us an email.\n\nLicense\n~~~~~~~\n\nThis python package is released under the permissive MIT license.\n\nContact\n~~~~~~~\nContact details are available at the staff pages of `Mark Teese`__ or `Philipp Heckmeier`__ within the `Langosch lab`__\nof the Technical University of Munich.\n\n.. _MarkWebsite: http://cbp.wzw.tum.de/index.php?id=49&L=1\n.. _PhilippWebsite: http://cbp.wzw.tum.de/index.php?id=55\n.. _LangoschWebsite: http://cbp.wzw.tum.de/index.php?id=9\n\n__ MarkWebsite_\n__ PhilippWebsite_\n__ LangoschWebsite_\n\n\n.. image:: https://raw.githubusercontent.com/teese/eccpy/master/docs/images/signac_seine_bei_samois.png\n   :height: 150px\n   :width: 250px\n\nExamples\n~~~~~~~~\n\n**fit to obtain r_max**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/aniso_savgol_fit.png\n   :height: 500 px\n   :width: 500 px\n\n**fit to obtain r_inf**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/aniso_seg2_fit.png\n   :height: 500 px\n   :width: 500 px\n\n**barchart comparing r_max**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/01_barchart_r_max.png\n   :height: 200 px\n   :width: 200 px\n\n**barchart comparing r_inf**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/02_barchart_r_inf.png\n   :height: 200 px\n   :width: 200 px\n\n**linechart comparing fit to full data for three samples**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/06_linechart_savgol.png\n   :height: 500 px\n   :width: 500 px\n\n**linechart comparing fit to r_inf for three samples**\n\n.. image:: https://raw.githubusercontent.com/teese/blitzcurve/master/blitzcurve/images/08_linechart_seg2.png\n   :height: 500 px\n   :width: 500 px\n\n",
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        "keywords": "fluorescence FRET rotation curve savitzky golay anisotropy time resolved TRAM single molecule protein bioinformatics biophysics microscopy",
        "license": "MIT",
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        "name": "blitzcurve",
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