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        "description": ".. image:: artwork/github.png\n   :alt: bcbio banner\n   :align: center\n\nValidated, scalable, community developed variant calling, RNA-seq and small RNA\nanalysis. You write a high level configuration file specifying your inputs and\nanalysis parameters. This input drives a parallel run that handles distributed\nexecution, idempotent processing restarts and safe transactional steps. bcbio\nprovides a shared community resource that handles the data processing component\nof sequencing analysis, providing researchers with more time to focus on the\ndownstream biology.\n\n.. image:: https://travis-ci.org/bcbio/bcbio-nextgen.png\n    :target: https://travis-ci.org/bcbio/bcbio-nextgen\n\nFeatures\n--------\n\n- Community developed: We welcome contributors with the goal of\n  overcoming the biological, algorithmic and computational challenges\n  that face individual developers working on complex pipelines in\n  quickly changing research areas. See our `users page`_ for examples\n  of bcbio-nextgen deployments, and the `developer documentation`_ for\n  tips on contributing.\n\n- Installation: `A single installer script`_ prepares all\n  third party software, data libraries and system configuration files.\n\n- `Automated validation`_: Compare variant calls against common reference\n  materials or sample specific SNP arrays to ensure call correctness.\n  Incorporation of multiple approaches for alignment, preparation and\n  variant calling enable unbiased comparisons of algorithms.\n\n- Distributed: Focus on `parallel analysis and scaling`_ to handle\n  large population studies and whole genome analysis. Runs on single\n  multicore computers, in compute clusters using `IPython parallel`_,\n  or on the Amazon cloud. See the `parallel documentation`_ for full\n  details.\n\n- Multiple analysis algorithms: bcbio-nextgen provides configurable\n  `variant calling, RNA-seq and small RNA pipelines`_.\n\n.. _IPython parallel: http://ipython.org/ipython-doc/dev/index.html\n.. _parallel documentation: https://bcbio-nextgen.readthedocs.org/en/latest/contents/parallel.html\n.. _A single installer script: https://bcbio-nextgen.readthedocs.org/en/latest/contents/installation.html#automated\n.. _users page: https://bcbio-nextgen.readthedocs.org/en/latest/contents/introduction.html#users\n.. _developer documentation: https://bcbio-nextgen.readthedocs.org/en/latest/contents/code.html\n.. _variant calling, RNA-seq and small RNA pipelines: https://bcbio-nextgen.readthedocs.org/en/latest/contents/pipelines.html\n.. _parallel analysis and scaling: http://bcb.io/2013/05/22/scaling-variant-detection-pipelines-for-whole-genome-sequencing-analysis/\n.. _Automated validation: http://bcb.io/2014/05/12/wgs-trio-variant-evaluation/\n\n\nQuick start\n-----------\n\n1. `Install`_ ``bcbio-nextgen`` with all tool dependencies and data files::\n\n         wget https://raw.github.com/bcbio/bcbio-nextgen/master/scripts/bcbio_nextgen_install.py\n         python bcbio_nextgen_install.py /usr/local/share/bcbio --tooldir=/usr/local \\\n           --genomes GRCh37 --aligners bwa --aligners bowtie2\n\n   producing an editable `system configuration file`_ referencing the installed\n   software, data and system information.\n\n2. `Automatically create a processing description`_ of sample FASTQ and BAM files\n   from your project, and a CSV file of sample metadata::\n\n         bcbio_nextgen.py -w template freebayes-variant project1.csv sample1.bam sample2_1.fq sample2_2.fq\n\n   This produces a `sample description file`_ containing pipeline `configuration options`_.\n\n3. Run analysis, distributed across 8 local cores::\n\n         cd project1/work\n         bcbio_nextgen.py ../config/project1.yaml -n 8\n\n.. _system configuration file: https://github.com/bcbio/bcbio-nextgen/blob/master/config/bcbio_system.yaml\n.. _sample description file: https://github.com/bcbio/bcbio-nextgen/blob/master/config/bcbio_sample.yaml\n.. _Automatically create a processing description: https://bcbio-nextgen.readthedocs.org/en/latest/contents/configuration.html#automated-sample-configuration\n.. _Install: https://bcbio-nextgen.readthedocs.org/en/latest/contents/installation.html#automated\n.. _configuration options: https://bcbio-nextgen.readthedocs.org/en/latest/contents/configuration.html\n\nDocumentation\n-------------\n\nSee the `full documentation`_ and `longer analysis-based articles\n<http://bcb.io>`_. We welcome enhancements or problem reports using `GitHub`_\nand discussion on the `biovalidation mailing list`_.\n\n.. _full documentation: https://bcbio-nextgen.readthedocs.org\n.. _GitHub: https://github.com/bcbio/bcbio-nextgen/issues\n.. _biovalidation mailing list: https://groups.google.com/d/forum/biovalidation\n\nContributors\n------------\n\n- `Miika Ahdesmaki`_, AstraZeneca\n- `Luca Beltrame`_, IRCCS \"Mario Negri\" Institute for Pharmacological Research, Milan, Italy\n- `Christian Brueffer`_, Lund University, Lund, Sweden\n- `Alla Bushoy`_, AstraZeneca\n- `Guillermo Carrasco`_, Science for Life Laboratory, Stockholm\n- `Nick Carriero <http://www.simonsfoundation.org/about-us/staff/staff-bios/#nick-carriero-ph-d>`_, Simons Foundation\n- `Brad Chapman`_, Harvard Chan Bioinformatics Core\n- `Saket Choudhary`_, University Of Southern California\n- `Peter Cock`_, The James Hutton Institute\n- `Matthias De Smet <https://github.com/matthdsm>`_, Center for Medical Genetics, Ghent University Hospital, Belgium\n- `Matt Edwards`_, MIT\n- `Mario Giovacchini`_, Science for Life Laboratory, Stockholm\n- `Karl Gutwin <https://twitter.com/kgutwin>`_, Biogen\n- `Jeff Hammerbacher`_, Icahn School of Medicine at Mount Sinai\n- `Oliver Hofmann <https://umccr.github.io/>`_, University of Melbourne Centre for Cancer Research\n- `John Kern <https://github.com/kern3020>`_\n- `Rory Kirchner`_, Harvard Chan Bioinformatics Core\n- `Tetiana Khotiainsteva <https://github.com/tetianakh>`_, Ardigen\n- `Jakub Nowacki`_, AstraZeneca\n- `John Morrissey <https://github.com/jwm>`_, Harvard Chan Bioinformatics Core\n- `Lorena Pantano <https://github.com/lpantano>`_, Harvard Chan Bioinformatics Core\n- `Brent Pedersen`_, University of Colorado Denver\n- `James Porter`_, The University of Chicago\n- `Valentine Svensson`_, Science for Life Laboratory, Stockholm\n- `Paul Tang`_, UCSF\n- `Stephen Turner`_, University of Virginia\n- `Roman Valls`_, Science for Life Laboratory, Stockholm\n- `Kevin Ying`_, Garvan Institute of Medical Research, Sydney, Australia\n- `Vlad Saveliev`_, Center for Algorithmic Biotechnology, St. Petersburg University\n\n\n.. _Miika Ahdesmaki: https://github.com/mjafin\n.. _Luca Beltrame: https://github.com/lbeltrame\n.. _Christian Brueffer: https://github.com/cbrueffer\n.. _Guillermo Carrasco: https://github.com/guillermo-carrasco\n.. _Alla Bushoy: https://github.com/abushoy\n.. _Brad Chapman: https://github.com/chapmanb\n.. _Peter Cock: https://github.com/peterjc\n.. _Mario Giovacchini: https://github.com/mariogiov\n.. _Rory Kirchner: https://github.com/roryk\n.. _Jakub Nowacki: https://github.com/jsnowacki\n.. _Brent Pedersen: https://github.com/brentp\n.. _James Porter: https://github.com/porterjamesj\n.. _Valentine Svensson: https://github.com/vals\n.. _Paul Tang: https://github.com/tanglingfung\n.. _Stephen Turner: https://github.com/stephenturner\n.. _Roman Valls: https://github.com/brainstorm\n.. _Kevin Ying: https://github.com/kevyin\n.. _Jeff Hammerbacher: https://github.com/hammer\n.. _Matt Edwards: https://github.com/matted\n.. _Saket Choudhary: https://github.com/saketkc\n.. _Vlad Saveliev: https://github.com/vladsaveliev\n\nLicense\n-------\n\nThe code is freely available under the `MIT license`_.\n\n.. _MIT license: http://www.opensource.org/licenses/mit-license.html\n",
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