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        "author": "Nigatu Ayele",
        "author_email": "naodm2006awet4@gmail.com",
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            "License :: OSI Approved :: MIT License",
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        "description": "MICTI- Marker gene Identification for Cell Type Identity\n========================================================\n\nRecent advances in single-cell gene expression profiling technology have revolutionized the understanding of molecular processes underlying developmental cell and tissue differentiation, enabling the discovery of novel cell types and molecular markers that characterize developmental trajectories.  Common approaches for identifying marker genes are based on pairwise statistical testing for differential gene expression between cell types in heterogeneous cell populations, which is challenging due to unequal sample sizes and variance between groups resulting in little statistical power and inflated type I errors. \n\nOverview\n--------\n\nWe developed an alternative feature extraction method, *Marker gene Identification for Cell Type Identity* (**MICTI**), that encodes the cell-type specific expression information to each gene in every single cell. This approach identifies features (genes) that are cell-type specific for a given cell-type in heterogeneous cell population.\n\n\nInstallation\n------------\n\nTo install the current release:\n\n\tpip install MICTI\n\nHow to use MICTI\n----------------\n\nImport MICTI:\n\n\tfrom MICTI import MARKER\n\nCreating MICTI object for known cell-type cluster label:\n\n\tmictiObject=MARKER.MICTI(datamatrix, geneName, cellName, cluster_assignment=cell_type, k=None, th=0, ensembel=False, organisum=\"hsapiens\")\n\n2D visualisation with tSNE:\n\n\tmictiObject.get_Visualization(dim=2, method=\"tsne\")\n\nGet MICTI marker genes:\n\n        cluster_1_markers=mictiObject.get_markers_by_Pvalues_and_Zscore(1, threshold_pvalue=.01,threshold_z_score=0)\n\nMarkers heatmap plots:\n\n\tmictiObject.heatMap()\n\nMarkers Radar plots:\n\n\tmictiObject.get_Radar_plot()\n\nGene Ontology enrichment analysis for cell-type marker genes in each of cell-type clusters\n\n\tenrechment_table=mictiObject.get_gene_list_over_representation_analysis(list(cluster_1_markers.index))\n\tenrechment_table #gene-list enrichment analysis result for the cell-type marker genes for cluster-1\n\n\nLicence\n-------\n\n[MICTI LICENCE](./LICENSE)\n\n\n",
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